Abstract
To study the absorption, metabolism, and excretion of medroxyprogesterone acetate in animals and man, a method was developed for measurement of the intact drug in plasma based on: (a) cyclohexane extraction of the specimen, (b) formation of the 3-enol heptafluorobutyrate ester, and (c) quantification via GLC using an electron-capture detector. The assay is quantitative above 1ng medroxyprogesterone acetate/ml of plasma, and overall precision is approximately ± 10% (SD) in the range of 5–20 ng/ml. The procedure was successfully applied to absorption studies in dogs after intramuscular drug administration. Plasma drug concentrations, as measured by radioimmunoassay, were five to 10 times greater as compared to the GLC assay, indicating that drug-related materials in addition to intact medroxyprogesterone acetate were in peripheral circulation.
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