Abstract
Plasma samples obtained from monkeys after oral administration of 0.5–2.0mg. of chlormadinone acetate were extracted with hexane. The hexane extracts were washed, concentrated, and analyzed by GLC using a 63Ni electron-capture detector. The analyses were performed on a 30.5−cm. (1‐ft.) column packed with either 3.8% methyl silicone gum rubber on silanized diatomite or a mixed phase consisting of 3.0% methyl silicone gum rubber and 1.5% polyethylene glycol 20,000 on silanized diatomite. Levels as low as 1.0ng. steroid/ml. plasma were detected by this procedure when 5-ml. samples of plasma were available.
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