Abstract
Cephaelis ipecacuanha shoots were rooted in different concentrations of auxins on solid Murashige and Skoog (Physiol Plant 15:473–497, 1962) media and liquid MS media with glass beads. Glass beads were used as an alternative source of support matrix in liquid culture media to replace agar. The highest rooting response was observed on liquid MS medium with glass beads containing 2 mg l−1 α-naphthalene acetic acid within 15–20 days. Shoot elongation was also found to be better compared to solid MS medium. Rooted shoots were successfully hardened and established in the soil. Hence, an easy, reliable and reproducible protocol was developed for in vitro rooting of Ipecac on liquid MS medium with glass beads, which is biologically inert and reusable.
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