Abstract
A novel HPTLC method has been developed for the estimation of glabridin in Licorice rhizome and its Unani polyherbal formulation (Qurs-e-Gul). Separation was achieved on silica using toluene, dichloromethane, and ethyl acetate in equal ratios. A compact, well resolved peak of glabridin with RF value 0.56 ± 0.02 was observed. Calibration curve revealed a good linear relationship with r2 value of 0.993 between the peak area and concentration in the range of 25–500 ng spot−1. The proposed method was validated as per the International Conference on Harmonization (ICH) guidelines. The stability assessment was carried out by studying degradation of glabridin stressed by acid, base, oxidation, thermal, and humidity. Photodegradation was also carried out after keeping the drug in sunlight, dark, and in UV lights. The method proposed can be used for routine determination of glabridin in crude drugs and in herbal formulations containing Licorice as one of the ingredients, for quality control as well as for stabilit...
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
