Gökkuşağı Alabalığının (Oncorhynchus mykiss) Sperm Kalitesinde Bilgisayarlı Otomatik Sperm Analiz Sisteminde (CASA) Leja ve Makler Lamlarının Performansının Karşılaştırılması

Abstract

In this study, the effect of the chamber used for the automated analysis of sperm motility and sperm kinematics parameters by CASA was evaluated of rainbow trout (Oncorhynchus mykiss) sperms. The assessment of motility parameters was carried out using CEROS II (Hamilton-Thorne, Beverly, MA, USA) connected to CX41 microscope (Olympus, Japan) at room temperature. Sperm samples were collected from five adult males by abdominal massage during the reproduction season and analyzed with two different chambers as follows specialty: Leja 2 cell chambered with 20 µl deep (Leja Products, Netherlands) and Makler chamber, round shape with 10 µl deep (Sefi-Medical Instrument, Haifa, Israel). Total sperm motility (Mot, %), and Velocity of Curvilinear (µm/s) were measured. For fertilization test, eggs from one female (550 g approximately 7500 eggs) were separated in equal five parts. Each part of eggs (approximately 1.500 eggs) was fertilized with each analyzed sperm (5ml). Fertilization, incubation procedure and calculation of fertilization rates have been kept as used routinely for rainbow trout culture procedure. The fertilization rates were found ˃80% for all used males. The motility percentage of samples analyzed by Leja has been found higher 90% while by makler changed between 26-45%. There is significantly effect on different chambers used in this study to determining the motility percentage. The high stability results and matched the fertilization success were detected in Leja 2-chamber. Statistical study with motility percentage showed a significant difference between Leja and Makler chambers (p<0,05).