Abstract
In this study, the humoral immune responses and host protection of rainbow trout, O. mykiss (Walbaum, 1792) against Ichthyophthirius multifiliis (Ich) were determined after vaccination with live theronts, sonicated trophonts, adjuvant added heat inactived theronts. Immunizations with live theronts, sonicated trophonts or adjuvant added heat inactive theronts by intraperitoneal (I.P.) injection. Serum immobilization titres were determined 21 days post-immunization. Four weeks after immunization, the number of trophonts were assessed in rainbow trout skin four days after challenge. Mean of serum immobilization titres levels were significantly higher (P<0.05) in fish immunized with live theronts (1/67,4) and sonicated trophonts (1/29,7) by I.P. injection, than in the fish immunized with adjuvant added heat inactivated theronts (1/11,4) and PBS (1/9,42) by I.P. injection. Mean number of the trophonts that can penetrated to skins were significantly lower (P<0.05) in fish administered live theronts (21 trophonts/fish) and sonicated trophonts (29,13 tr/fish) by i.p. injection, than in the fish immunized with adjuvant added heat inactivated theronts (48,53 tr/fish) and PBS (54,07 tr/fish) by I.P. injection. Therefore, partial protection was noted on those fish. There was found positive correlation between higher levels of immobilization titres and partial protection in the immunized fish.
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