Giving early solids to infants. Beware of misclassifying eczema.

Abstract

<h3>Abstract</h3> Recently, a novel purple-pericarp super-sweetcorn line, ‘Tim1’ (<i>A1A1</i>.<i>sh2sh2</i>) is derived from the purple-pericarp maize ‘Costa Rica’ (<i>A1Sh2</i>.<i>A1Sh2</i>) and white <i>shrunken2</i> (<i>sh2</i>) super-sweetcorn ‘Tims-white’ (<i>a1sh2</i>.<i>a1sh2</i>), however information regarding purple colour controlling anthocyanin biosynthesis genes and sweetcorn gene is lacking. Specific sequence differences in the CDS (coding DNA sequence) and promoter regions of the anthocyanin biosynthesis structural genes, <i>anthocyanin1</i> (<i>A1</i>), <i>purple aleurone1</i> (<i>Pr1</i>) and regulatory genes, <i>purple plant1</i> (<i>Pl1</i>), <i>plant colour1</i> (<i>B1</i>), <i>coloured1</i> (<i>R1</i>), and the sweetcorn structural gene, <i>shrunken2</i> (<i>sh2</i>) were investigated using the publicly available annotated yellow starchy maize, B73 (NAM5.0) as a reference genome. In the CDS region, the <i>A1, Pl1</i> and <i>R1</i> gene sequence differences of ‘Tim1’ and ‘Costa Rica’ were similar, as they control purple-pericarp pigmentation, however the <i>B1</i> gene showed similarity between the ‘Tim1’ and ‘Tims-white’ lines, which may indicate that it does not have a role in controlling pericarp colour, unlike a previous study. In case of <i>Pr1</i> gene, unlike ‘Costa Rica’, 6- and 8-bp dinucleotide (TA) repeats were observed in the promoter region of the ‘Tims-white’ and ‘Tim1’ lines, respectively, indicating the defective functionality (redder colour in ‘Tim1’ than purple in ‘Costa Rica’) of the recessive <i>pr1</i> allele. In the sweetcorn structural gene (<i>sh2</i>), sequence similarity was observed between purple-sweet ‘Tim1’ and its white-sweet parent ‘Tims-white’, as both display a shrunken phenotype in their mature kernels. These findings revealed that the developed purple-sweet line is different than the reference yellow-nonsweet line regarding both the anthocyanin biosynthesis and sweetcorn genes.