Abstract
BackgroundAlthough tibial plateau fracture is an uncommon injury, its regulation is challenging and there are some influencing factors, including the effects of severe bone displacement, depression and cancellous bone cartilage, and inevitable cartilage damage. And GIT1 plays an important role in bone mass and 78 osteoblast cell migration.MethodsThe study used 72 C57/BL6 mice. A tibial plateau fracture model was established by using mice with the same number of GIT1 gene deletions (the experimental group) and their wild-type littermates (the control group). Joint and bone callus recovery were evaluated by X-ray and CT thin layer scans. Micro CT assay and histomorphometry were conducted in order to evaluate the volume of newly formed blood vessels. Type II collagen expression in tibial tissues after tibial plateau fracture were detected by immunohistochemistry after 7, 14 and 21 days. The number of proliferating cell nuclear antigen (PCNA) positive cells after tibial plateau fracture was tested by immunohistochemistry after 14 and 21 days. The terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling (TUNEL) staining was conducted after 14 and 21 days in order to test chondrocyte apoptosis in tibial tissues after tibial plateau fracture.ResultsThe GIT1 gene deletion group mice spent less time on the rotating rod than the control group mice (P < 0.05). Compared with the control group, postoperative recovery was retarded, because GIT1 gene deletion slowed down neovascularization after tibial plateau fracture (P < 0.05). Compared with the control group, mouse type II collagen expression significantly decreased in the GIT1 gene deletion group, and the proportion of PCNA positive cells significantly decreased (P < 0.05). The TUNEL results indicate that GIT1 gene deletion led to reduced chondrocyte apoptosis.ConclusionGIT1 gene deletion can inhibit chondrocyte proliferation and apoptosis during the recovery of tibial plateau fracture, so as to delay chondrocyte differentiation and tibial plateau fracture healing.
Highlights
Tibial plateau fracture is an uncommon injury, its regulation is challenging and there are some influencing factors, including the effects of severe bone displacement, depression and cancellous bone cartilage, and inevitable cartilage damage
We hypothesize that Gkinase interacting proteins 1 (GIT1) gene deletion may delay differentiation of chondrocyte and healing of tibial plateau fracture through suppressing proliferation and apoptosis of chondrocyte
Behavioral observation of mice and results of rotarod test On the 14th day after operation, mice in the control group were less active in the cage, and right fracture hind limbs of these mice were raised and were unable to touch the ground without external help, while mice in the experimental group were almost inactive in the cage
Summary
Tibial plateau fracture is an uncommon injury, its regulation is challenging and there are some influencing factors, including the effects of severe bone displacement, depression and cancellous bone cartilage, and inevitable cartilage damage. GIT1 plays an important role in bone mass and 78 osteoblast cell migration. Tibial plateau fractures are thought of as uncommon injuries, and are not frequently reported in literature [1]. The regulation of tibial plateau fractures is challenging and there are some. GIT1 plays an important role in bone mass and osteoblast cell migration, in which the structure of GIT1 protein could adhere to osteoblasts by inhibiting P-ERK1/2 [16, 17]. We hypothesize that GIT1 gene deletion may delay differentiation of chondrocyte and healing of tibial plateau fracture through suppressing proliferation and apoptosis of chondrocyte
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