Abstract
The activation of a DNA polymerase 5 (pol 5) purified from bovine placenta by ginsenosides from Panax Ginseng C.A. Meyer has been studied. Preincubation of the enzyme with ginsenosides increased the polymerase activity 2.2-fold in a dose-dependent manner. There was a reproducible decrease in K m, in addition to a substantial increase in V max, in response to increasing concentrations of ginsenosides. Ginsenosides also activated the proofreading ability of 3′- to 5′-exonuclease activity associated with DNA pol δ. The coordinated activation of both polymerase and exonuclease activities of DNA pol 5 by ginsenosides is consistent with the view that its polymerase and its exonuclease activities residue on the same protein molecule. UV/Vis difference spectroscopic studies suggested that the activation of DNA pol 5 by ginsenosides might be due to the conformational change induced by ginsenosides binding.
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