Ginsenoside-Rh2 Inhibits Proliferation and Induces Apoptosis of Human Gastric Cancer SGC-7901 Side Population Cells.

Jun Qian,Bo Xie,Chen-Xu Guo,Xin Jin,Jing Li,Da-Jun Yu,Jian-Guang Jia,Li-Yu Qian

doi:10.7314/apjcp.2016.17.4.1817

Abstract

To observed the effects of ginsenoside -Rh2 (GS-Rh2) on proliferation and apoptosis of side population (SP) human gastric cancer SGC-7901 cells. SGC-7901 SP and Non-SP cells were sorted by flow cytometry and assessed using the cck-8 method. Expression of apoptosis-related proteins Bax and Bcl-2 of SP before and after the intervention was determined by Western-blotting. It was found that the proliferation of SP was significantly faster than that of NSP (<0.05). In addition, GS-Rh2 inhibited proliferation of gastric cancer SP cells, induced cell cycle arrest and cell apoptosis, and changed the expression of BAX/Bcl-2 proteins in a time-dependent and concentration-dependent manner (<0.05). With increase of GS-Rh2 dose, GS-Rh2 gradually inhibit the proliferation of SGC-7901 SP cells, which have high proliferation rate, through G1/G0 phase arrest, followed by apoptosis which involves the up-regulation of Bax and the down-regulation of Bcl-2.

Highlights

Gastric cancer is the most commonly diagnosed gastrointestinal cancer with high recurrence rate, high metastatic rate and resistance to chemotherapeutic drugs (Saricanbaz et al, 2014)
With increase of ginsenoside -Rh2 (GS-Rh2) dose, GS-Rh2 gradually inhibit the proliferation of SGC-7901 side population (SP) cells, which have high proliferation rate, through G1/G0 phase arrest, followed by apoptosis which involves the up-regulation of Bax and the down-regulation of Bcl-2
Reagents and instruments Cell culture medium RPMI-1640 and 0.25% trypsin were purchased from Hyclone (Logan, UT, USA); Penicillin-Streptomycin, Liquid was purchased from Invitrogen (Carlsbad, CA, USA); fetal bovine serum (FBS) was purchased from Tianhang Biological Technology, (Hangzhou, Zhejiang, China); Hoechst 33342 and verapamil were obtained from Sigma (Saint Louis, MO, USA); GS-Rh2 was purchased from Sigma; the

Summary

Introduction

Gastric cancer is the most commonly diagnosed gastrointestinal cancer with high recurrence rate, high metastatic rate and resistance to chemotherapeutic drugs (Saricanbaz et al, 2014). The biological characteristics of gastric cancer cells are investigated mainly through studying proliferation (Kato et al, 2012), apoptosis (Akagi et al, 2013), nude mice tumorigenic (Zheng et al, 2010) and protein molecular level of gastric cancer cells (Wang et al, 2014). Ginseng is one of the commonly used Chinese traditional medicines that showed anti-cancer activity in clinical studies (Nakata H et al, 1998). The research proved that the mainly active ingredients of anti-tumor in ginseng is ginsenosides (Choi S et al, 2011; Tang XP et al, 2013) , through the comparison of various kinds of ginsenoside found that GS-Rh2 has the strongest ability to inhibited the proliferation of cancer cells (Li B et al, 2011).

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