Abstract
Endometritis is the inflammatory response of the endometrial lining of the uterus and is associated with low conception rates, early embryonic mortality, and prolonged inter-calving intervals, and thus poses huge economic losses to the dairy industry worldwide. Ginsenoside Rb1 (GnRb1) is a natural compound obtained from the roots of Panax ginseng, having several pharmacological and biological properties. However, the anti-inflammatory properties of GnRb1 in lipopolysaccharide (LPS)-challenged endometritis through the TLR4-mediated NF-κB signaling pathway has not yet been researched. This study was planned to evaluate the mechanisms of how GnRb1 rescues LPS-induced endometritis. In the present research, histopathological findings revealed that GnRb1 ameliorated LPS-triggered uterine injury. The ELISA and RT-qPCR assay findings indicated that GnRb1 suppressed the expression level of pro-inflammatory molecules (TNF-α, IL-1β and IL-6) and boosted the level of anti-inflammatory (IL-10) cytokine. Furthermore, the molecular study suggested that GnRb1 attenuated TLR4-mediated NF-κB signaling. The results demonstrated the therapeutic efficacy of GnRb1 in the mouse model of LPS-triggered endometritis via the inhibition of the TLR4-associated NF-κB pathway. Taken together, this study provides a baseline for the protective effect of GnRb1 to treat endometritis in both humans and animals.
Highlights
Endometritis is considered a major global problem and has been associated with a decline in the reproductive performance of animals [1,2]
The present research was planned to explore the beneficial effects of Ginsenoside Rb1 (GnRb1) in the murine model of endometritis caused by LPS
A histopathological score was recorded according to the previous study [17] (Figure 2F). These findings show that GnRb1 efficiently ameliorated LPS-stimulated endometritis
Summary
Endometritis is considered a major global problem and has been associated with a decline in the reproductive performance of animals [1,2]. The postpartum involution period is very crucial regarding the infection of the endometrium caused by bacteria, Molecules 2021, 26, 7089. Molecules 2021, 26, 7089 Molecules 2021, 26, 7089 riod is very crucial regarding the infection of the endometrium caused by bacteria, e2spofe cially Gram-negative (G-ve) bacteria [3]. Among these G-ve bacteria, Escherichia coli (E. coli) is known as the major bacterial etiology of endometritis [4,5]. The present research was planned to explore the beneficial effects of GnRb1 in the murine model of endometritis caused by LPS
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
