Gingival Crevicular Fluid as a Novel Potential Source of Biomarkers Distinguishes Pubertal from Post-Pubertal Subjects.

Xi Wen,Yan Gu,Feng Chen

doi:10.3390/diagnostics6040041

Xi Wen, Yan Gu + Show 1 more

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https://doi.org/10.3390/diagnostics6040041

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Journal: Diagnostics	Publication Date: Nov 17, 2016
Citations: 4	License type: CC BY 4.0

Affiliation: Peking University, Stomatology Hospital

Abstract

Detection of pubertal growth peak is vital in orthodontic treatment timing and planning. Gingival crevicular fluid (GCF) contains abundant proteins from different sources and has been proven to be an ideal source of biomarkers. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF/MS) is an advanced technique that can detect low-molecular-weight peptides with high sensitivity and resolution. The aim of this research was to identify novel candidate biomarkers in GCF to help the diagnosis of pubertal growth peak by MALDI-TOF/MS. Results showed that the peak intensities of six peptides were significantly different between two groups: 1660.2 Da, 1783.0 Da, 2912.5 Da, 4178.6 Da, 5064.9 Da, and 6108.9 Da and are considered to be potential candidate biomarkers to identify pubertal growth peak. Further studies are needed to identify sequence information of these candidate biomarkers.

Highlights

Accurate identification of different phases of bone maturation plays a vital role in the decision of orthodontic treatment timing in growing patients with skeletal malocclusion
Recent studies showed that new possibilities may be provided by biomarkers in gingival crevicular fluid (GCF) [5,6,7]
The aim of this research was to identify novel candidate biomarkers in GCF to help the diagnosis of pubertal growth peak with the employment of MALDI-TOF/MS, and lay the foundation for future research

Summary

Introduction

Accurate identification of different phases of bone maturation plays a vital role in the decision of orthodontic treatment timing in growing patients with skeletal malocclusion. Chronological age and dental age have been proved to be unreliable in identifying growth stage [1,2]. X-ray-based hand-wrist analysis and cervical vertebral maturation (CVM) are the most frequently used methods in clinics. These methods are considered to have extra X-ray radiation and not good reproducibility due to the experience of clinicians [3,4]. Stable and noninvasive biomarkers are needed to help the diagnosis of pubertal growth peak. Recent studies showed that new possibilities may be provided by biomarkers in gingival crevicular fluid (GCF) [5,6,7]

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