Abstract
Generate Indexes for Libraries (GIL)is a software tool for generating primers to be used in the production of multiplexed sequencing libraries. GIL canbe customized in numerous ways to meetuser specifications, including length, sequencing modality, color balancing, and compatibility with existing primers, and produces ordering and demultiplexing-ready outputs. GIL is written in Python and is freely available on GitHub under the MIT license: https://github.com/de-Boer-Lab/GIL and can be accessed as a web-application implemented in Streamlit at https://dbl-gil.streamlitapp.com.
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