Abstract
C-banding, by Giemsa staining, is largely restricted to distal regions of rye chromosomes. This applies, also, to B chromosomes. The distribution of C-bands coincides with that of distally localised heterochromatin. The area of metaphase chromosomes staining with Giemsa corresponds to the area occupied by heterochromatin in interphase nuclei as revealed by Feulgen staining. The Giemsa-stained C-bands account, therefore, for all the heterochromatin in rye nuclei. Individual chromosomes within the complement are readily identified following Giemsa staining.
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