Abstract
Precise control of generative organ development is of great importance for the productivity of crop plants, including legumes. Gibberellins (GAs) play a key role in the regulation of flowering, and fruit setting and development. The major repressors of GA signaling are DELLA proteins. In this paper, the full-length cDNA of LlDELLA1 gene in yellow lupine (Lupinus luteus L.) was identified. Nuclear-located LlDELLA1 was clustered in a second phylogenetic group. Further analyses revealed the presence of all conserved motifs and domains required for the GA-dependent interaction with Gibberellin Insensitive Dwarf1 (GID1) receptor, and involved in the repression function of LlDELLA1. Studies on expression profiles have shown that fluctuating LlDELLA1 transcript level favors proper flower and pod development. Accumulation of LlDELLA1 mRNA slightly decreases from the flower bud stage to anther opening (dehiscence), while there is rapid increase during pollination, fertilization, as well as pod setting and early development. LlDELLA1 expression is downregulated during late pod development. The linkage of LlDELLA1 activity with cellular and tissue localization of gibberellic acid (GA3) offers a broader insight into the functioning of the GA pathway, dependent on the organ and developmental stage. Our analyses provide information that may be valuable in improving the agronomic properties of yellow lupine.
Highlights
Interest in the cultivation of legumes, including lupine (Lupinus L.), is growing significantly
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Gibberellins play a crucial role in the development of generative organs [38]
Summary
Interest in the cultivation of legumes, including lupine (Lupinus L.), is growing significantly. The high fodder value of lupine, universal consumption values, and its role in sustainable and ecological production are being widely accepted and appreciated [1]. A key problem in growing lupine in rapidly changing environmental conditions is premature and excessive generative organ abortion, which significantly reduces the yield. This effectively discourages the cultivation of this species by innovative farmers who expect large and stable yields every year. It is extremely important to understand the basic mechanisms responsible for the formation, growth, and development of lupine flowers and pods at the genetic, molecular, and hormonal levels
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