Abstract
The present report describes intracellular signaling events elicited by gibberellin (GA) in barley aleurone, where the phytohormone induces an increase on phosphoinositides turnover in a time-dependent manner. A biphasic behavior for PtdInsP was demonstrated by changes in [32P]PtdInsP levels, the earlier phase peaking at 5 min and the second phase at 25 min while PtdInsP2 turnover was rapid but transient, peaking only at 5 min. In contrast, the structural phospholipid PtdCho turnover was only affected after 40 min. In addition, GA stimulated the synthesis of all phospholipids. Incorporation of [32P]Pi in unstimulated aleurone showed that both synthesis and degradation of polyphosphoinositides were relatively faster than those of structural phospholipids. We discuss the possible reasons why the changes in synthesis and turnover of phosphoinositide are required for GA signaling pathway and why it may be a critical event in the control of secretion in aleurone.
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