Gibberellic Acid Alleviates Cadmium-Induced Seed Germination Inhibition through Modulation of Carbohydrate Metabolism and Antioxidant Capacity in Mung Bean Seedlings

Abstract

Gibberellins (GA) are the decisive players in seed germination whose functionality could be adversely affected by the presence of cadmium (Cd); however, the underlying mechanisms are unclear. Eco-toxicological effects of Cd (0, 25, 50, 75, and 100 µM) on the early stages of ontogenesis in a mung bean variety (ML-2056) were investigated. Seed germination characteristics along with Cd-tolerance index were recorded at the seventh day of germination. Additionally, endogenous gibberellic acid (GA3) level, amylase activity, oxidative stress, and the antioxidant defense system were also investigated in Cd-stressed germinating seedlings. Results revealed that Cd reduced seed germination and interfered with GA synthesis in a concentration-dependent manner. Further, to validate the role of GA in Cd-tolerance, experiments were executed to explore the effect of seed priming with GA3 and its biosynthesis inhibitor paclobutrazol (PBZ) on ML-2056 under Cd stress. Application of GA3 improved the activities of amylase and carbohydrate-metabolizing enzymes, the antioxidant defense system, and sustained lower H2O2 and TBARS contents, whereas PBZ caused a significant reduction in growth and decreased endogenous GA3 content in Cd-stressed ML-2056, suggesting a crucial role of GA synthesis in reversing Cd-induced negative effects. Overall, GA synthesis played a crucial role in mitigating Cd toxicity in mung bean, which might be used as a criterion for developing Cd-stress-tolerant genotypes.