Abstract
Alkaline phosphatase (IAP) is a marker of intestinal microvillus membrane. Changes in IAP activity have been studied as a function of Giardia lamblia ( G. lamblia) infection using rat as the experimental model. At day 11 and 15 post-infection, enzyme activity was reduced ( p < 0.01) compared to controls. The enzyme levels were essentially similar to control values by day 30 post-infection. Analysis of the enzyme activity in cell fractions across crypt–villus axis revealed a marked decrease in enzyme activity in the villus tip and mid villus regions but a considerable increase ( p < 0.01) in enzyme activity in the crypt base of 11 day post-infected animals compared to that in controls. The observed changes in IAP activity were confirmed by assaying the enzyme activity in acrylamide gels using bromo-chloro-indolyl phosphate staining under non-denaturing conditions. These findings indicate differential changes across the crypt–villus axis, but impaired alkaline phosphatase levels in G. lamblia infected rat intestine.
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