Giant Viruses of Amoebas: An Update.

Sarah Aherfi,Bernard La Scola,Philippe Colson,Didier Raoult

doi:10.3389/fmicb.2016.00349

Abstract

During the 12 past years, five new or putative virus families encompassing several members, namely Mimiviridae, Marseilleviridae, pandoraviruses, faustoviruses, and virophages were described. In addition, Pithovirus sibericum and Mollivirus sibericum represent type strains of putative new giant virus families. All these viruses were isolated using amoebal coculture methods. These giant viruses were linked by phylogenomic analyses to other large DNA viruses. They were then proposed to be classified in a new viral order, the Megavirales, on the basis of their common origin, as shown by a set of ancestral genes encoding key viral functions, a common virion architecture, and shared major biological features including replication inside cytoplasmic factories. Megavirales is increasingly demonstrated to stand in the tree of life aside Bacteria, Archaea, and Eukarya, and the megavirus ancestor is suspected to be as ancient as cellular ancestors. In addition, giant amoebal viruses are visible under a light microscope and display many phenotypic and genomic features not found in other viruses, while they share other characteristics with parasitic microbes. Moreover, these organisms appear to be common inhabitants of our biosphere, and mimiviruses and marseilleviruses were isolated from human samples and associated to diseases. In the present review, we describe the main features and recent findings on these giant amoebal viruses and virophages.

Highlights

Viruses were first described at the end of the nineteenth century as ultrafilterable and submicroscopic infectious agents (Beijerinck, 1898; Loeffler and Frosch, 1898)
A. polyphaga mimivirus (APMV), the pioneer representative of giant viruses of amoebas, was isolated in 1992 from a water sample collected in England in an air-conditioning system, while investigating a pneumonia outbreak (Table 1; Figures 1–5; La Scola et al, 2003)
Marseillevirus genomes are 350–380 kilobp large, with a G+C content of ≈45% and harbor genes they might have shared with bacteria, archaea, viruses, and eukaryotes, including amoebas (Aherfi et al, 2014)

Summary

Introduction

Viruses were first described at the end of the nineteenth century as ultrafilterable and submicroscopic infectious agents (Beijerinck, 1898; Loeffler and Frosch, 1898). As of 2015, five new or putative families of viruses infecting amoebas were described, which encompass members from giant viral families Mimiviridae and Marseilleviridae, pandoraviruses, faustoviruses, and mimivirus virophages, and Pithovirus sibericum and Mollivirus sibericum represent type strains of putative new giant virus families (La Scola et al, 2008; Desnues and Raoult, 2012; Colson et al, 2013a,d; Philippe et al, 2013; Legendre et al, 2014, 2015; Reteno et al, 2015).

Results

Conclusion