棉花盐胁迫途径中蛋白磷酸化同源基因(GhSOS2)2种剪接体的克隆及表达分析

Abstract

【Objective】 The objective of this study is to understand the salt stress related genes expression and clone the key salt-tolerance genes under the conditions of salt tolerance in cotton. 【Method】 Through screening of the cotton EST databases and integration of the objective EST sequences, a homologous gene named GhSOS2 of plasma membrane Na(superscript +)/H(superscript +) reverse transporter protein phosphorylation was obtained by RT-PCR method, respectively, under the conditions of non-salt and salt stress treatment of Zhongmiansuo 49. Meanwhile, the expression characteristics were analyzed by real time quantitative PCR method. 【Result】 The sequence analysis showed that there were two splice forms of GhSOS2a (GenBank accession number: GU188960) and GhSOS2b (GenBank accession number: GU188961), encoding 445 and 421 amino acid residues, respectively. Compared to GhSOS2b, an extra exon of 72 bp exists in GhSOS2a due to different splicing ways. Real time quantitative PCR analysis showed that GhSOS2a was expressed in the mixture tissue of root and stem, leaf tissue under both non-salt and salt stress conditions. However, GhSOS2b was expressed in the mixture tissue of root and stem, leaf tissue only under the conditions of salt stress. Furthermore, its expression was much higher than that of the GhSOS2a. 【Conclusion】 GhSOS2 gene was alternatively spliced into two kinds of splice forms under the condition of salt stress in cotton. The splice form GhSOS2b may be mainly involved and play an important role in the pathway of cotton plasma membrane Na(superscript +)/H(superscript +) reverse transporter.