Abstract
Ghrelin (GRLN) stimulates maturational gonadotropin (LH) secretion in goldfish. This study identified GRLN receptors (GHS-Rs) in goldfish tissues and examined the involvement of voltage-sensitive Ca 2+ channels (VSCCs) in ghrelin action. A partial goldfish GHS-R1a sequence was obtained and expression observed in brain, pituitary, spleen, kidney, heart, gill, ovary, testis, and intestine. Synthetic goldfish GRLN (gGRLN 19) stimulated LH secretion from dispersed goldfish pituitary cells in column perifusion and increased intracellular Ca 2+ ([Ca 2+] i) in identified goldfish gonadotropes. gGRLN 19 did not stimulate LH secretion either in the presence of Ca 2+-free media, or the L-type VSCC inhibitors nifedipine and verapamil. Similarly, gGRLN 19-elicited increases in [Ca 2+] i were attenuated by Ca 2+-free media and nifedipine. Furthermore, when LH release and [Ca 2+] i were elevated by Bay K8644, gGRLN 19 had no further effect. These results indicate that GHS-R1a is present in goldfish pituitary and Ca 2+ entry through VSCC mediates direct gGRLN 19 action on LH release in goldfish pituitary cells.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
