Abstract
Hepatocellular carcinoma (HCC) is a common malignant liver tumor worldwide. Tumor recurrence and metastasis contribute to the bad clinical outcome of HCC patients. Substantial studies have displayed lncRNAs modulate various tumorigenic processes of many cancers. Our current work was aimed to investigate the function of LINC00675 in HCC and to recognize the potential interactions between lncRNAs and microRNAs. GFI1 can exhibit a significant role in the progression of human malignant tumors. Firstly, GFI1 was identified using real-time PCR in HCC tissues and cells. In this work, we indicated GFI1 was remarkably reduced in HCC tissues and cells. Meanwhile, GFI1 specifically interacted with the promoter of LINC00675. Up-regulation of LINC00675 obviously repressed the migration and invasion capacity of SMCC-7721 and QGY-7703 cells in vitro. Moreover, decrease of LINC00675 competitively bound to miR-942-5p that contributed to the miRNA-mediated degradation of GFI1, thus facilitated HCC metastasis. The ceRNA function of LINC00675 in HCC cells was assessed and confirmed using RNA immunoprecipitation assay and RNA pull-down assays in our work. Additionally, we proved overexpression of miR-942-5p promoted HCC progression, which was reversed by the up-regulation of GFI1. In summary, LINC00675 might act as a prognostic marker for HCC, which can inhibit HCC development via regulating miR-942-5p and GFI1.
Highlights
Hepatocellular carcinoma (HCC) is a frequent malignant tumor and it is the third common cause of cancer-related death across the world [1, 2]
We identified LINC00675 was aberrantly expressed in human HCC tissues and cells
The data of our present study displayed that Growth factor independent 1 transcriptional repressor (GFI1)/ LINC00675/miR-942-5p as a prognostic factor in HCC development
Summary
Hepatocellular carcinoma (HCC) is a frequent malignant tumor and it is the third common cause of cancer-related death across the world [1, 2]. In spite of the therapeutic treatment for HCC, its survival rate is still poor because of the recurrence after surgery [3, 4]. Novel insights into the mechanism of HCC are in need to recognize the prognostic molecular markers to improve HCC patient survival [5]. Growth factor independent 1 transcriptional repressor (GFI1) is located in chromosome 1p22 in the human genome [6]. GFI1 can act as a transcriptional repressor by interacting with other cofactors [7]. It has been reported that GFI1 exhibits an important role in hematopoietic stem cells. GFI1 inhibits proliferation and preserves functional integrity in regulating self-renewal of hematopoietic stem cells [8]. The functional role of GFI1 in HCC carcinogenesis has not been fully investigated
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