Gezieltes Entfernen von Fluorochromkonjugaten nach der Zellsortierung

Abstract

Flow cytometric analysis and sorting rely on fluorescent labeling with multiple antibody-fluorochrome conjugates. Currently available conjugates remain on the cells after flow sorting and thus can limit downstream applications. In contrast, REAlease® conjugates enable specific cell labeling and can be completely removed from the epitopes after the experiment; cell epitopes and fluorescence channels that were blocked through labeling become available for subsequent experiments.