Gewinnung haploider Stammzellkulturen der Maus für genetische Screens

Abstract

Haploid mouse embryonic stem cells are a handy tool to investigate loss of function mutations. Furthermore, these cells can contribute to chimeric embryos and can be used to introduce genetic modifications into mouse germ lines. Nevertheless, haploid cell cultures have to be maintained by flow sorting on a regular basis due to spontaneous diploidization. Here we present physical differences between haploid and diploid cells, which can be used in applied cell sorting of haploid cell cultures.