Gestational diabetes alters the expression of genes involved in Sertoli cells maturation in testis tissue from adult rat offspring

Abstract

Introduction: Previous Studies indicate that both type 1 and 2 diabetes mellitus have adverse effects on the male reproductive system. Furthermore, it has recently shown that induced gestational diabetes significantly reduces the Sertoli cells number in the rat offspring. This study was done to evaluate the effect of gestational diabetes mellitus (GDM) on the expression of genes involved in Sertoli cell proliferation and maturation in the adult rat offspring. Material and Methods: To test this hypothesis, 12 Wistar rat dams were randomly allocated to control and diabetic groups. The diabetic group received 40 mg/kg/body weight of streptozotocin on day 0 of gestation. After delivery, six offspring of each group at the age of 12 weeks scarified and testis tissue harvested. After RNA extraction, the expression of p27kip1, A-kinase anchoring protein 9, CX43, and aromatase genes in both groups was evaluated by quantitative real-time polymerase chain reaction. Results: Our data showed that the expression of all examined genes which are important in Sertoli cells maturity and function were lower in GDM offspring. p27kip1 and aromatase were significantly downregulated in GDM offspring by 57% and 41%, respectively (P * 0.05*). Discussion and Conclusion: In summary, we provide evidence that GDM may adverse effects on the male reproductive system in the offspring by alterations in the expression of genes responsible for Sertoli cell proliferation and differentiation.