Germline-specific labeling of the somatic chromosomes by protein phosphatase 2A and histone H3S28 phosphorylation in Acricotopus lucidus

Abstract

Additional chromosomes limited to the germline (=Ks) were established as a special form of germline–soma differentiation in the Orthocladiinae, a subfamily of the Chironomidae (Bauer and Beermann in Z Naturforsch 7b: 557–563, 1952). The Ks together with the somatic chromosomes (=Ss) pass through a complex chromosome cycle with elimination at mitosis and a monopolar migration of all Ks. The dissimilar behavior of Ks and Ss in these exceptional mitoses initiated the search for differential chromosome marks in the orthocladiid Acricotopus lucidus. The search, using immunofluorescence, revealed that in metaphases of male gonial mitoses, and both meiotic divisions, the Ss are fully labeled by protein phosphatase 2A (PP2A) and histone H3S28ph, while in metaphases of somatic cells both marks were detected only at the centromeres of the Ss. In another orthocladiid, Psectrocladius obvius, the same labeling pattern of the Ss as in A. lucidus was established for H3S28ph, but not for PP2A, which was localised solely at the centromeres. In Chironomus nuditaris, a species possessing no Ks, PP2A and H3S28ph signals were always restricted to the centromeres. High levels of H3K4me3, a marker of transcriptionally competent chromatin, were detected on the Ss in metaphases I of C. nuditaris, while in both orthocladiids, the Ss in metaphases I were devoid of H3K4me3 signals. This strongly supports an earlier idea of a silencing of the Ss in male meiosis of A. lucidus suggesting the possibility of extending this concept to the Orthocladiinae. The germline–soma differentiation in A. lucidus is not only made apparent by the occurrence of Ks but also by a germline-specific labeling of the Ss by PP2A and H3S28ph.