Germination Responses of Ryegrass (Annual vs. Perennial) Seed to the Interactive Effects of Temperature and Salt-Alkali Stress.

Jixiang Lin,Xiufeng Yan,Xiaoyu Hua,Bolin Dong,Xiaoyuan Peng

doi:10.3389/fpls.2018.01458

Jixiang Lin, Xiufeng Yan + Show 3 more

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https://doi.org/10.3389/fpls.2018.01458

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Abstract

Ryegrass is considered a useful grass species for forage production and turf purposes. Annual ryegrass (Lolium multiflorum Lam.) and perennial ryegrass (Lolium perenne L.)are two species of ryegrass with similar genomes. So far, little information exists concerning their physiological response to salt-alkali stress during germination stage, especially under different temperature regimes. Seeds of ryegrass were germinated at four alternating temperatures (10–20, 15–25, 20–30, and 25–35°C) with salinity (NaCl) and alkalinity (Na2CO3, high pH) stresses. Results showed that optimal germination for both species under stress conditions occurred at higher temperatures (20–30°C for annual ryegrass; 20–30°C and 25–35°C for perennial ryegrass). Germination percentage and germination rate were both inhibited by increasing salinity or alkalinity, particularly higher alkalinities under any temperature. The inhibitory effects of the high salinity on germination were greater at 10–20°C for both species. However, seeds were subjected to more stress at 25–35°C under alkali stress even though the concentration was very low. In addition, both high and low temperatures lead to a markedly decrease in seed germination under alkali stress for perennial ryegrass. Recovery percentage of both species were highest at 400 mM salinity and 25 mM alkalinity under any temperature, especially 10–20°C, and 25–35°C also resulted in lower recovery percentages under both stresses for ryegrass. Moreover, annual ryegrass had a much higher recovery percentage than perennial ryegrass under such stress conditions. These results suggest that salinity stress and alkalinity stress are greatly different, and the salt-alkaline tolerance of ryegrass seeds is greatly affected by the interactions of temperature and salinity–alkalinity.

Highlights

Soil salinization and alkalization have been considered as major environmental threats to the entire terrestrial ecosystem, which inhibit plant growth and lead to a further soil degradation (Lin J. et al, 2014; Guo et al, 2015)
Three-way analysis of variance (ANOVA) results showed that seed germination percentage of ryegrass was affected by ryegrass species, temperature, salinity and their interactions (P < 0.001, Table 1)
The highest germination percentage of both species was obtained in non-stress condition, and no significant difference was observed between different temperatures

Summary

Introduction

Soil salinization and alkalization have been considered as major environmental threats to the entire terrestrial ecosystem (e.g., forest, grassland, agricultural and urban), which inhibit plant growth and lead to a further soil degradation (Lin J. et al, 2014; Guo et al, 2015). In China, approximately 7 million hectares (ha) of land area are affected by salinity. Germination Responses of Ryegrass to Temperature and Stress and semi-arid areas of northeastern China, alkalinized land has exceeded 70% of the total area and is still expanding (Wang et al, 2011). Seed germination is the initial and one of the most pivotal stages in plant life cycle (Bhatt and Santo, 2016). In natural habitats, seed germination and the following seedling establishment phases are always affected by many environmental factors such as soil salinity–alkalinity, light, temperature fluctuations and water availability (Keblawy et al, 2017). Temperature and soil salinity–alkalinity are considered to be the main limiting factors during germination stage for most plant species in the Northeast of China (Lin and Tang, 2005)

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