Abstract
The germination of sclerotia of Macrophomina phaseolina from 3- to 20-day-old PDA cultures incubated at 32 °C and 24 °C varied from 98 to 25% and 75 to 30%, respectively. Germinability of sclerotia from cultures of all ages tested was rapidly lost during storage in desiccators for 24 h at 6 °C or in screw-top vials at room temperature for 30 days. Germination of sclerotia stored in screw-top vials at 6 °C for 30 days declined less rapidly. There was a linear relationship between the size of the sclerotium and the number of germ tubes emerging from it. The role of sclerotium size in survival and what factors may be responsible for loss of germinability with increasing age of source culture are discussed.
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