Germ-line versus somatic cells. I. Stereological study of differentiating embryonic tissues of Tetrodontophora bielanensis (Hexapoda, Collembola)

Abstract

We examined five different somatic tissues and compared them with germ-line cells to verify the "disposable soma" theory. Two embryonic stages and second-stage juveniles of Tetrodontophora bielanensis Waga, 1842 (Hexapoda, Collembola) were studied. Our results show that changes in relative volume of mitochondria during differentiation of cells correlate well with transformations of cell morphology. During morphological transformation of differentiating somatic cells, the relative volume of mitochondria in their cytoplasm is high, whereas in the differentiated tissues, this parameter is much lower. Surprisingly, the highest value of relative volume density of mitochondria is found in the cytoplasm of germ-line cells. If we accept that this parameter indicates the cell metabolism rate, then our results should be taken as supporting the "disposable soma" theory. It is also conceivable that the higher volume of mitochondria in the germ-line cells have nothing to do with energy production but, for instance, with the production or function of nuage material (germ-cell determinant) in the germ-line cells. These two possibilities are discussed.