Geographical and Ethnic Differences Influence Culturable Commensal Yeast Diversity on Healthy Skin

Cheryl Leong,Bettina Schmid,Joyce Wang,Martin Glatz,Antony Sagayaraj Irudayaswamy,Min Jet Toi,Joleen Peh Zhen Goh,Thomas L Dawson,Philipp P Bosshard

doi:10.3389/fmicb.2019.01891

Abstract

Commensal fungi such as Malassezia, Candida, and Rhodotorula are common on healthy skin but are also associated with opportunistic invasive and superficial infections. Skin microbial community characterization has been extensively performed worldwide, with a focus on the 16S bacterial community. These studies have focused on geographically distinct or targeted cohorts with variable reported species distributions of commensal yeast species. To determine the effects of extrinsic environmental factors such as geography, climate, and ethnicity on detected healthy skin commensal yeast diversity, we compared cohorts from Singapore and Zürich, Switzerland, representative of two geographically and climatically distinct regions comprising multi-ethnic (Chinese, Malay, Indian, Caucasian) and predominantly white Caucasian cohorts, respectively, using identical skin sampling and culture methods. We chose to use a culture-based approach as cultures isolated from patients are still required for studies of pathogenicity and antifungal susceptibility. Detection of yeast species by culture-dependent and independent sequencing-based methods suggest healthy skin diversity reflects a species distribution representative of the geography, climate and ethnic background of their local populations. Culture success and species diversity was also found to be dependent on climate, with warm tropical climates favoring high positive culture rates and greater species diversity. Multilocus sequence typing data suggests some strains are geographically distinct and may be used to segregate potential disease-causing commensals. For accurate collection and characterization of skin microbial communities, it remains recommended to employ a combination of culture-dependent and sequence-based culture-independent methods. Characterization of healthy mycobiomes in geographically distinct local populations will be useful in defining the role of commensal fungi in health and disease.

Highlights

Yeasts are an integral part of the skin microbial communities and play an important role in skin health and disease (Findley et al, 2013)
M. globosa, M. furfur, M. restricta, M. sympodialis, and Malassezia dermatis Are Common in Singapore
Fewer species of Malassezia were detected on the scalp, with M. globosa (47.6%, 20 of 42) and M. restricta (23.8%, 10 of 42) being the dominant colonizing species respectively (Figure 1A and Table 1)

Summary

Introduction

Yeasts are an integral part of the skin microbial communities and play an important role in skin health and disease (Findley et al, 2013). Malassezia, a lipid dependent yeast, is the dominant resident fungal species in human adults and accounts for up to 80% of skin fungal genomes (Gao et al, 2010). Studies suggest that many infectious Candida and Malassezia strains have commensal origins (Chang et al, 1998; Kam and Xu, 2002). This underscores the potential role of commensal yeasts as opportunistic pathogens. Culturedependent methods are still required for studies of pathogenicity and antifungal susceptibility (Leong et al, 2017), our culture-based approach

Methods

Results

Conclusion