Abstract
Dengue viruses (DENVs) cause the greatest public health burden globally among the arthropod-borne viruses. DENV transmission risk has also expanded from tropical to subtropical regions due to the increasing range of its principal mosquito vector, Aedes aegypti. Focal outbreaks of dengue fever (dengue) in the state of Florida (FL) in the USA have increased since 2009. However, little is known about the competence of Ae. aegypti populations across different regions of FL to transmit DENVs. To understand the effects of DENV genotype and serotype variations on vector susceptibility and transmission potential in FL, we orally infected a colony of Ae. aegypti (Orlando/ORL) with low passage or laboratory DENV-1 through -4. Low passage DENVs were more infectious to and had higher transmission potential by ORL mosquitoes. We used these same DENVs to examine natural Ae. aegypti populations to determine whether spatial distributions correlated with differential vector competence. Vector competence across all DENV serotypes was greater for mosquitoes from areas with the highest dengue incidence in south FL compared to north FL. Vector competence for low passage DENVs was significantly higher, revealing that transmission risk is influenced by virus/vector combinations. These data support a targeted mosquito-plus-pathogen screening approach to more accurately estimate DENV transmission risk.
Highlights
IntroductionPublisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations
In pairwise comparisons of Dengue viruses (DENVs)-exposed ORL mosquitoes, midgut infection rates (IRs) was highest for DENV-1 H with 97%, followed by DENV-4 H (61%), DENV-2
We provided evidence that field Ae. aegypti populations from different DENV risk zones in FL vary in their vector competence profiles between the four DENV serotypes, and between low passage and laboratory virus strains
Summary
Publisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations. Dengue viruses (DENVs) belong to the genus Flavivirus in the family Flaviviridae Ae. There are four genetically related dengue viruses termed DENV-1, DENV-2, DENV-3, and DENV-4. There are four genetically related dengue viruses termed DENV-1, DENV-2, DENV-3, and DENV-4 These arthropod-borne viruses (arboviruses) are transmitted to humans through the bite of an infected Aedes species mosquito. The primary vector of these viruses is Aedes aegypti (Ae. aegypti). Dengue viruses are the causative agents of dengue fever (“dengue”)
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