Abstract
Electrical impedance spectroscopy is a soft analysis methods for living cell cultures, that is non-destructive for cell cultures and allows continuous measurement of cellular changed in real-time. In this work we report on an in vitro impedimetric sensors that measures the confluence and the density of human epithelial cell layers. A mechanically inflicted damage in a confluent cell layer leads to a reduction of the impedance, while the healing of the cell layer was closing the holes and increased impedance. The entire healing process could be tracked in real time via the impedance. To advance existing in vitro cell impedance spectroscopy we have not only used opwn metal electrodes but also electrically insulated interdigitated microelectrodes. The influence of the insulation on sensor performance is experimentally and theoretically analyzed A circuit model was established. In a comparison between open (not passivated) electrodes was made. The best setup for sensitivity and repeatability, which are crucial parameters for cell analysis, will be presented. The presented novel sensor design allows for the integration of alternative electrode materials such as aluminum. This enables cost-effective fabrication of large-volume sensor arrays.
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