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Abstract
Genotyping of the Holstein-Friesian crossbred cattle for CD18 gene using PCR-RFLP
Highlights
All the 50 animals under present investigation were found to be normal as the amplified PCR product upon digestion with Taq I restriction enzyme, revealed two bands of 313 bp and 54 bp for normal animals
Recent reports suggest that the mutant gene has already been observed in the HF crossbred cattle population of India, which makes it necessary to screen the animals to avoid the risk of spreading Bovine Leucocyte Adhesion Deficiency (BLAD) in the breeding cattle population
Bovine Leucocyte Adhesion Deficiency (BLAD) is a Holstein-Friesian (HF) specific autosomal recessively inherited disorder characterized by recurrent pneumonia, ulcerative and granulomatous stomatitis, enteritis, periodontitis, loss of teeth, delayed wound healing, persistent neutrophilia and death at an early age [1]
Summary
Bovine Leucocyte Adhesion Deficiency (BLAD) is a Holstein-Friesian (HF) specific autosomal recessively inherited disorder characterized by recurrent pneumonia, ulcerative and granulomatous stomatitis, enteritis, periodontitis, loss of teeth, delayed wound healing, persistent neutrophilia and death at an early age [1]. BLAD is a disease characterized by a reduced expression of the adhesion molecules on neutrophils, called ß2 integrins (a complex of the CD11/CD18 family of proteins that are structurally and functionally related to glycoproteins). The mutation leads to a CD18 protein with impaired function. The carrier frequency of BLAD among US Holstein cattle was found to be 15% among breeding bulls and 8% among cows [2]. Use of breeding HF bulls imported from the USA in many countries lead to a high incidence of BLAD carriers in their native/crossbred cattle population including India [3,4,5,6,7,8]
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