Genotyping of T2D susceptible genes in a high risk North-East Indian population

Abstract

ObjectiveSeveral variants have been reported in certain populations but cannot be reproduced in other populations and this leads to a new domain of challenge in exploring, evaluating and replicating several gene variants particularly to identify the common and novel type 2 diabetes associated variations. This study aims to find out the possible role and contribution of selected gene variants for T2D susceptibility in tribal Mizo population group. MethodsGenotyping of the variants LRRC31,HLA-G, VEGFA, CD2AP, ELMO1, TERF1, TCF7L2 and FTO was performed using AgenaMass ARRAY platform for 384 T2D cases and 384 healthy controls. The data was analyzed using population based case control association study. The clinical assessment was carried using standard measurement and their relationship with the gene variants was analyzed using One- Way ANOVA. ResultsSignificant association between the variantsVEGFA (rs2010963, rs3025020), ELMO1 (rs741301, rs1882080), CD2AP (rs9369717) and FTO (rs9939609) and T2D was observed. These variants are observed to be significantly associated with the clinical parameters like fasting blood glucose, Hb1AC and postprandial blood glucose. In contrast, the variants, LRRC31 (rs16847897), HLA-G (rs1063320), CD2AP (rs9369717), TERF1 (rs6982126) and TCF7L2 (rs290475) do not show genetic association. ConclusionVEGFA, CD2AP, FTO and ELMO1 gene variants were found to be associated with T2D. This is the first report of association of the clinical parameters with the genetic variants and also for the high incidence of T2D in a tribal population from Northeast India which may contribute in understanding the missing heritability of T2D using candidate gene association studies.