Genotyping of Mx Gene Related to Avian Influenza (AI) Using PCR-RFLP Analysis on KUB Chicken

Abstract

Mx gene is antiviral molecules which is part of the innate immune response. In chicken, antiviral activity against vesicular stomatitis virus and the H5N1 virus has been reported to be dependent on the presence of asparagine (A) at position 631 of the Mx protein. The objective of this study was to detect the genotype of Mx gen on KUB chicken as a basis to do molecular selection on natural antibody trait. A total of 120 KUB chicken were used in this study. DNA fragment of Mx gene was amplified and analyzed using PCR-RFLP method. Three types of restriction enzymes (RsaI, SspI and HpaI) were used to determine Mx gene genotype. The results showed that the frequency of the Mx gene genotype on KUB chicken was polymorphic. The AA genotype frequencies resulted from RsaI, SspI and HpaI restriction enzymes were 0.50, 0.425 and 0.283, respectively. While the AG genotype frequencies were 0.40, 0.492 and 0.634; and GG genotypes were 0.10, 0.083 and 0.083, respectively. The frequency of A and G alleles were 0.657 and 0.343. KUB chicken population in this study remained at Hardy-Weinberg equilibrium and three types of restriction enzyme could be used to detect Mx gene.