Genotyping of Insulin growth Factor-1 (IGF-1) gene at SNP g.5752G>C on Lakor goat from Southwest Maluku Regency

Abstract

Lakor goat is an indigenous breed of Indonesia from Southwest Maluku regency, Indonesia. They are known to have a good adaptation to high temperatures and poor feed quality. Their meat is one of the protein supplies for the Lakor island local community. Genetic and environmental factors influence growth in animals. Insulin Growth Factor-1 (IGF-1) plays a substantial part in postnatal development, skeletal growth, cell proliferation, and metabolism in the animal. This study aimed to identify the genotype pattern of the IGF-1 gene SNP g.5752 G>C of Lakor goat from Southwest Maluku using the PCR-RFLP method. Sixty-three samples were collected from three villages in Lakor island. The follicle hair samples were taken from the tail and then isolated using a DNA extraction kit. Genotyping of IGF-1 gene SNP g.5752 G>C used PCR-RFLP method with HaeIII restriction enzyme. Results showed that all samples have BB genotype patterns or are monomorphic. It might be caused by the isolation or restricted area in Lakor island and the utilization of extensive rearing systems. This mutation in SNP g.5752 G>C of the IGF-1 gene was not used as a candidate of marker selection in the Lakor goat population.