Genotyping of amino acid-producing Corynebacterium glutamicum strains based on multi-locus sequence typing (MLST) scheme

Junjie Yang,Sheng Yang,Yimeng Kong

doi:10.1186/s40643-014-0030-8

Abstract

Confusing parental information may hinder to dissect mechanisms of amino acid hyper-producing Corynebacterium glutamicum strains. Thus, an efficient method for genotyping of the C. glutamicum is heavily called. Multi-locus sequence typing (MLST) is currently the most popular molecular typing technique. But currently this method is not available for C. glutamicum. In this study, a MLST scheme was established based on sequences of seven housekeeping genes, for genotyping of C. glutamicum. The MLST method performed an efficient discrimination of 17 strains and helps to understand the population structure of this bacterium. This work has expanded the MLST method to C. glutamicum and developed an efficient technique to discriminate strains of uncertain origin.

Highlights

Confusing parental information may hinder to dissect mechanisms of amino acid hyper-producing Corynebacterium glutamicum strains
Due to roughly identical 16S rDNA sequences, many independent strains previously regarded as Brevibacterium and Corynebacterium species in the Corynebacteriaceae family have been categorized into the C. glutamicum species, especially Brevibacterium lactofermentum, Brevibacterium flavum, and Corynebacterium acetoacidophilum [3,4,5]
The retrieved 16S rDNA sequences are almost identical, with a similarity >99% compared with the sequence of the C. glutamicum ATCC 13032, indicating that all of the strains belong to C. glutamicum [3,4]

Summary

Introduction

Confusing parental information may hinder to dissect mechanisms of amino acid hyper-producing Corynebacterium glutamicum strains. Non-spore-forming Gram-positive bacteria Corynebacterium glutamicum are widely used in amino acid production industry with numerous ideal attributes [1,2]. Due to roughly identical 16S rDNA sequences, many independent strains previously regarded as Brevibacterium and Corynebacterium species in the Corynebacteriaceae family have been categorized into the C. glutamicum species, especially Brevibacterium lactofermentum, Brevibacterium flavum, and Corynebacterium acetoacidophilum [3,4,5]. Confusing parental information may hinder to dissect. Compared with other molecular typing techniques, multi-locus sequence typing (MLST) is currently the most popular one [7,8]. MLST relies on allelic variants in conserved genes to calculate phylogenetic relationship of strains. Each strain is assigned with an allelic profile or sequence type (ST).

Objectives

Methods

Results

Conclusion