Abstract
The present study examined the prevalence and genotypes of Cryptosporidium andersoni in cattle in Shaanxi province, China. A total of 2071 fecal samples (847 from Qinchuan cattle and 1224 from dairy cattle) were examined for the presence of Cryptosporidium oocysts, and 70 samples (3.4%) were C. andersoni-positive and those positive samples were identified by PCR amplification of the small subunit ribosomal RNA (SSU rRNA) and the Cryptosporidium oocyst wall protein (COWP) genes. C. andersoni was the only species found in the examined cattle in this province. Fifty-seven C. andersoni isolates were characterized into 5 MLST subtypes using multilocus sequence typing analysis, including a new subtype in the native beef breed Qinchuan cattle. All of these C. andersoni isolates presented a clonal genetic structure. These findings provide new insights into the genetic structure of C. andersoni isolates in Shaanxi province and basic data of Cryptosporidium prevalence status, which in turn have implications for controlling cryptosporidiosis in this province.
Highlights
Cryptosporidium spp. infect a wide range of hosts including humans and cattle [1,2]
Cryptosporidium species and genotypes were determined by nested PCR amplification of the small subunit ribosomal RNA (SSU rRNA) gene [16] and by single PCR amplification of the Cryptosporidium oocyst wall protein (COWP) gene [30]
Phylogenetic analysis based on SSU rRNA and COWP gene sequences were conducted to identify species of Cryptosporidium isolates in the present study
Summary
Cryptosporidium spp. infect a wide range of hosts including humans and cattle [1,2]. Previous studies around the world have shown that cattle is the most common species of mammals known to be infected with Cryptosporidium spp., and is the main source of human infection with Cryptosporidium [2,3,4]. In China, Cryptosporidium infection has been reported in dairy cattle in Guangxi [23], Anhui [24], Henan [6,25] and Qinghai provinces [26], and in beef cattle in Anhui [27], Qinghai [26], Inner Mongolia [28] and other provinces. These studies mainly focused on prevalence and identification of Cryptosporidium species. The subtypes of C. andersoni in Qinchuan cattle and dairy cattle in this province were characterized using MLST
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