Abstract
BackgroundCanine parvovirus (CPV) is an important pathogen that causes acute enteric disease in dogs. It has mutated and spread throughout the world in dog populations. We provide an update on the molecular characterization of CPV that circulated in Nanjing, a provincial capital in China between 2009 and 2012.ResultsSeventy rectal swab samples were collected from the dogs diagnosed with CPV infection in 8 animal hospitals of Nanjing. Sequence analysis of VP2 genes of 31 samples revealed that 29 viral strains belonged to CPV-2a subtype, while other two strains were classified into CPV-2b. To investigate the pathogenicity of the prevalent virus, we isolated CPV-2a and performed the animal experiment. Nine beagles were inoculated with 105.86 of 50% tissue culture infectious doses (TCID50) of the virus. All the experimentally infected beagles exhibited mild to moderate mucoid or watery diarrhea on day 4 post-infection (p.i.). On day 9 p.i., characteristic histopathological lesions were clearly observed in multiple organs of infected dogs, including liver, spleen, kidney, brain and all segments of the small and large intestines, while viral DNA and antigen staining could be detected in the sampled tissues. It is notable that canine parvovirus was isolated in one from two brain samples processed.ConclusionOur results indicated that CPV-2a is the predominant subtype in Nanjing of China. And this virus caused extensive lesions in a variety of tissues, including the brain.
Highlights
Canine parvovirus (CPV) is an important pathogen that causes acute enteric disease in dogs
To provide an update on the molecular characterization of CPV that circulated in Nanjing, China, in the study, we characterized canine parvovirus from fecal samples of domestic dogs by polymerase chain reaction followed by sequencing, isolated the prevalent virus and performed in vivo experiments to investigate the pathogenicity of this virus
Prevalence and genetic characterization of canine parvovirus One DNA band of the expected size (583 bp), corresponding to the partial amplification of the VP2 gene, was observed by gel electrophoresis in all the 70 samples diagnosed with CPV infection
Summary
Canine parvovirus (CPV) is an important pathogen that causes acute enteric disease in dogs. It has mutated and spread throughout the world in dog populations. The canine parvovirus (CPV) infection is a viral illness that most commonly affects puppies. The infected dogs develop an acute gastroenteritis characterized by loss of appetite, vomiting, fever, diarrhea (from mucoid to haemorrhagic) and leucopenia [1]. VP2 is the major capsid protein, and it plays an important role in determining viral host ranges and tissue tropisms [3]. The virus emerged as dog pathogen in the late 1970's as host variant of feline panleukopenia virus (FPV) [5,6].
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