Abstract
BackgroundAcinetobacter baumannii strains with multiple antimicrobial resistance are primarily known as opportunistic nosocomial bacteria but they may also be regarded as emerging bacterial contaminants of food samples of animal origin. Here we aimed to study the molecular characteristics of the A. baumanni strains isolated from raw meat samples.MethodsA total of 22 A. baumanni strains were isolated from 126 animal meat samples and were genotyped by ERIC-PCR method and by PCR detection of their virulence and antimicrobial resistance determinants. A. baumannii strains with 80% and more similarities were considered as one cluster.ResultsSixteen different genetic clusters were found amongst the 22 A. baumanni strains. Of the 22 strains, 12 (54.54%) had similar genetic cluster. A. baumannii strains exhibited the highest percentage of resistance against tetracycline (90.90%), trimethoprim (59.09%), cotrimoxazole (54.54%) and gentamicin (50.00%). TetA (81.81%), tetB (72.72%), dfrA1 (63.63%), aac(3)-IV (63.63%), sul1 (63.63%) and aadA1 (45.45%) were the most commonly detected antibiotic resistance genes. FimH (81.81%), afa/draBC (63.63%), csgA (63.63%), cnf1 (59.09%), cnf2 (54.54%) and iutA (50.00%) were the most commonly detected virulence factors. A. baumannii strains isolated from the chicken meat samples had the highest similarities in the genetic cluster.ConclusionsA. baumannii strains with similar genetic cluster (ERIC-Type) had the same prevalence of antibiotic resistance, antibiotic resistance genes and virulence factors. Genetic cluster of the A. baumannii strains is the main factor affected the similarities in the genotypic and phenotypic properties of the A. baumannii strains.
Highlights
Acinetobacter baumannii strains with multiple antimicrobial resistance are primarily known as opportunistic nosocomial bacteria but they may be regarded as emerging bacterial contaminants of food samples of animal origin
A. baumannii strains with 80% and more than 80% similarity in the molecular typing were considered as one cluster
In conclusion, we studied the phenotypic and genotypic patterns of antibiotic resistance of the A. baumannii strains isolated from meat samples of different animals
Summary
Acinetobacter baumannii strains with multiple antimicrobial resistance are primarily known as opportunistic nosocomial bacteria but they may be regarded as emerging bacterial contaminants of food samples of animal origin. A. baumannii strains isolated from clinical infections harbored the high prevalence of certain virulence factors including pathogenicity islands (PAI), P fimbriae (pap genes), S fimbriae adhesion and F1C (sfa), a fimbriae adhesion and Dr antigen family (afa/dra), type 1 fimbriae (fimH), fibronectin receptor (fbn), serum resistance (traT), curli fibers (csg), aerobactin (iutA), yersiniabactin (fyuA), polysaccharide coatings as group II and III capsules (kpsMT), cytotoxic necrotizing factors (cnf), invasins factors (IbeA) and colicin V production (cvaC). These factors are responsible for adhesion, colonization and invasion of the A. baumannii strains into the different target organs [12, 13]. This method has sporadically been used to characterize A. baumannii strains isolated from clinical samples [17]
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