Genotyping and Chi-Square Analysis of 967 bp Leptin Gene in Bligon Goat

Abstract

Leptin gene has contribution to affect the economical function in livestock such as growth rate and reproduction. The aim of the study was to identify the genetic marker related to growth and reproduction base on single nucleotide polymorphism in Leptin gene as a basic data for genotyping in Bligon goat. Thirty blood sample of Bligon goat have been extracted for DNA analysis. The target sequence of Leptin gene was amplified by polymerase chain reaction method. Four single nucleotide polymorphisms were detected at position g.758G/A, g.864C/T, g.1171G/A, dan g.1454G/A. Three out of four SNP can be used for genotyping. Two genotypes at those loci were found in this study. Genotype CC at locus g.864C/T have greater amount than genotype CT. The same result was also for genotype GG at both locus g. 1171G/A and g.1454G/A have higher number than the genotype AG. Frequency of allele C (0.97) and allele G (0.97) at Bligon goat population was higher than allele T (0.03) and alel A (0.03). The chi-square analysis shows that the population of Bligon goat still in genetics equilibrium with the value X2=0,849. This study provided the basic information for genotyping of local goat in Indonesia based on Leptin gene. The association of the genotype in Leptin gene with growth and reproduction trait can be the next agenda research in Bligon goat.