Genotyping and Antifungal Susceptibility of C. albicans Isolated from Infected women

Abstract

The aim of this study was to determine the genotypic and antifungal susceptibility of C. albicans obtained from vulvaginities women The study included 100 vulvaginitis patients who exhibited candidiases infection in uterus. vaginal swabs from women patients and healthy persons screened for the occurrence of C. albicans. Isolates were identified by the conventional mycological methods. Genotypes were determined using 25S rDNA PCR analysis. Vaginal C. albicans was detected in(39.6) % women patients and For the 21 isolates of C. albicans: 14 (66.7 %), 4 (19 %), and 3 (14.3%) isolates were recognized as genotypes A, B and C, respectively …in vitro susceptibility tests were performed using the agar inhibition zone method recommended by the Clinical and Laboratory Standard, the results showed that all C. albicans genotypes (100)%were sensitive to Miconazole. In the case of Nystatin (95)% of isolates were sensitive and (4.8)% were resistant. For ketoconazole (71.4)% of isolates were sensitive (23.8)%were resistant, and (4.8)%showed dose dependent sensitivity. For Fluconazole antifungal (66.7) % of isolates showed sensitivity to fluconazole, (28.6)% were resistant and (4.7)%were dose dependent.