Genotypic variation among 20 rice cultivars/landraces in response to cadmium stress grown locally in West Bengal, India

Abstract

Cadmium (Cd) is a hazardous soil contaminant and causes environmental toxicity when present beyond the allowable limit in soil. It can alter growth and metabolism in both plants and animals even at very low concentration. Being sessile in nature, plants try to evade this harmful effect by adopting various defence mechanisms including activation of antioxidants and other metal homeostasis mechanisms. This study shows the varietal Cd stress tolerance capacity of rice cultivars commonly grown in West Bengal, which is a rice biodiversity region in India. Seven days old rice (Oryza sativa L.) seedlings were treated with 10 μM CdCl2 for another 7days and different physiological and biochemical stress parameters were studied to compare the varietal stress responses. Principle component analysis (PCA) and root tolerance index (RTI) revealed that rice cultivars I.E.T-4786, Jamini and Netiya, Maharaj showed divergent stress responses towards susceptibility and tolerance. Histochemical localization of hydrogen peroxide (H2O2), superoxide (O2˙-) and pot experiment were performed in these four cultivars (I.E.T-4786-Jamini and Netiya-Maharaj) to elucidate the different Cd stress tolerance. Histochemical analysis, agronomic traits and grain Cd content analyses showed that I.E.T-4786 and Jamini were susceptible with no Cd accumulation in grain, whereas cultivars Netiya and Maharaj were stress tolerant and Cd accumulators. In addition, health risk assessment was monitored for dietary intake of Cd through Cd accumulating rice and non Cd accumulating rice genotypes were identified. Thus, the study identified the Cd tolerant and sensitive cultivars grown locally.