Genotypic investigation of drugs resistance and biofilm genes among ciprofloxacin-resistant Pseudomonas aeruginosa isolated from burn patients

Abstract

Burn patients are the serious targets of hospital-acquired infection occurred by Pseudomonas aeruginosa ( P. aeruginosa) , which is a main cause of burn patients morbidity and mortality, so this study aimed to molecular investigation of genes associated with antibiotic resistance among ciprofloxacin-resistant Pseudomonas aeruginosa. Current study involved 168 burn patients involved 107(63.69%) and 61 (36.3%) were females and male respectively, the results showed 132(78.57%) gram negative bacteria and 27(16.0%) gram positive bacteria while 9(5.3%) no bacterial growth. The results of Vitek-2 compact system recorded 75(44.64%) as P. aeruginosa isolates. Results of ciprofloxacin susceptibility showed 29(38.67%), while 34(45.33%) and 12(16%) of isolates were intermediate and sensitive respectively. Congo red agar assay was applied in current study for qualitative evaluation of pathogenic biofilm were 23/29 (79.31%), and 6/26(20.86%) as high, and moderate biofilm producer among isolates respectively. Result of Polymerase chain reaction (PCR) showed that gyrB was recorded among ciprofloxacin-resistant P. aeruginosa at rate 28/29 (96.55%). At same respect. results of efflux pump genes for oqxA and oqxB genes were detected among this pathogen at rate 26/29 (89.65%) and 29/29(100%) respectively. outer membrane genes, revealed that mexR gene was 27/29 (93.1%), while the spreading of the oprD gene was 26/29(89.65%). Results of molecular detection about colistin resistance genes revealed that the mcr-3 gene was 8/29 (27.58%), but genes of mcr-1 and mcr-2 were no detected in this work, Finally, results showed that 23/29 (79.31%) of ciprofloxacin-resistant P. aeruginosa were harbored pml gene.