Abstract

Three genotypes of warm-season adapted sorghum grain have been developed. The objectives of this study were to systematically investigate the magnitude differences in detailed chemical and nutrient profiles, protein subfractions, energy value, rumen degradation, intestinal digestibility of rumen undegraded protein as well as protein-related molecular structures in these genotypes of warm-season adapted sorghum grain (Sorghum bicolor L. Moench). The protein molecular structures were revealed by using vibrational non-invasive ATR-FTIR molecular spectroscopy. The results showed that the physicochemical, nutritional, and molecular structural features among three genotypes of warm-season adapted sorghum grain were different (P < 0.05). These sorghum genotypes had markedly (P < 0.05) differences in the protein-related molecular structures, including amides spectral intensity area, height and their intensity ratios. The results indicated that the molecular spectral profiles collected by non-invasive ATR-FTIR molecular spectroscopy could be used as a potential approach to predict nutrient flow and utilization in ruminant systems.

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