Genotypic correlation between Salmonella Enteritidis isolates from broiler breeders and hatchery flocks

İnci Başak Müştak,Hamit Kaan Müştak,Seyyide Sarıçam İnce

doi:10.2478/acve-2021-0033

İnci Başak Müştak, Hamit Kaan Müştak + Show 1 more

Open Access

https://doi.org/10.2478/acve-2021-0033

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Journal: Acta Veterinaria	Publication Date: Dec 1, 2021
License type: CC BY 4.0

Affiliation: Ankara University

Abstract

Abstract In this study, Salmonella Enteritidis strains isolated from dust and environmental materials from different flocks located in Turkey’s Western Black Sea region were examined by pulsed field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). A total of 59 S. Enteritidis strains isolated from broiler breeder and hatchery flocks, and one S. Enteritidis strain isolated from a stool sample of a farm worker were examined. PFGE analysis revealed two major PFGE groups and nine different macro restriction profiles. It was determined that 85% (51/60) of the strains were close to each other and comprised Group I. All S. Enteritidis strains had the same sequence type (ST): ST11. Isolation of strains with a single genotype suggests that there may be a cross transmission between the flocks.

Highlights

Salmonella enterica serovars are one of the main causes of foodborne infections in humans [1,2]
Other Salmonella serovars used for exclusion were in different clusters (B-E)
Enteritidis strains isolated from boiler breeder and hatchery flocks

Summary

Introduction

Salmonella enterica serovars are one of the main causes of foodborne infections in humans [1,2]. Intestinal colonization of chickens by Salmonella and excretion through feces leads to gastroenteritis in humans, especially by entering the food chain [3]. The most important food sources of Salmonella infections are meat, milk and eggs obtained from chickens [4]. Salmonella contamination has been a continuous problem since the 1900s, especially in the poultry industry. For this reason, Salmonella control programs are being implemented in many countries [5,6]. Enteritidis strains can be transmitted to humans from chickens and eggs [9]. In 2017, Salmonella was found in 3.31% of broiler flocks while the isolation rate from fresh broiler meat served as prepared food was 4.85% [10]. Enteritidis in broiler chickens was 9% [11]

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