Genotypic Characterization of Coagulase-negative Staphylococci Isolated from Sheep Milk in Slovakia

Ivana Pilipčincová,Milan Trávniček,Eva Dudriková,Mangesh Bhide

doi:10.2754/avb201079020269

Ivana Pilipčincová, Milan Trávniček + Show 2 more

Open Access

https://doi.org/10.2754/avb201079020269

Copy DOI

Abstract

Hitherto very few reports are available presenting identification and molecular characterization of the coagulase negative staphylococci (CNS) from sheep milk in the subclinical stage of mastitis. Furthermore, very scanty data are available on the epidemiological status of CNS in different Slovak provinces. Milk samples from 54 sheep farms located in eastern Slovak region were screened. A total 240 CNS were identified with series of biochemical testes (STAPH-API) and subjected further for genotyping with the help of pulse field gel electrophoresis (PFGE). The most frequently occurring CNS species according the biochemical characterization were:S. epidermidis(36.3 %),S. caprae(21.3 %),S. hominis(6.6 %),S. chromogenes(6.3 %),S. xylosus(5.8 %),S. warneri(5.0 %) andS. capitis(4.6 %). Further PFGE-based characterization of these isolates revealed six pulsotypes of theS. epidermidis, two ofS. caprae, three ofS. chromogenes, nine ofS. hominis, five ofS. capitisand seven ofS. xylosus. These results contribute to knowledge of the epidemiological situation of the CNS from the subclinical form of mastitis in Slovakia.

Highlights

Clinical and subclinical mastitis presents potential health hazard due to the increase in total bacterial count in milk (Fthenakis and Jones 1990; Gonzalo et al 2006)
A total 240 coagulase negative staphylococci (CNS) were identified with series of biochemical testes (STAPH-API) and subjected further for genotyping with the help of pulse field gel electrophoresis (PFGE)
Further PFGE-based characterization of these isolates revealed six pulsotypes of the S. epidermidis, two of S. caprae, three of S. chromogenes, nine of S. hominis, five of S. capitis and seven of S. xylosus. These results contribute to knowledge of the epidemiological situation of the CNS from the subclinical form of mastitis in Slovakia

Summary

Introduction

Clinical and subclinical mastitis presents potential health hazard due to the increase in total bacterial count in milk (Fthenakis and Jones 1990; Gonzalo et al 2006). CNS may sensitize and increase susceptibility of mammary cells to other infections CNS produce few virulence factors; they can cause infections in healthy host tissue. They are opportunists and adhere to metal devices to produce a protective biofilm. The ability to resist antimicrobials and produce biofilm enables CNS to persist on metal devices, milking equipments as well as on the milker’s hands, which serve a major source of staphylococcal spread

Objectives

Methods

Results

Conclusion