Genotypic and phenotypic study of E. coli isolated from children suffering from severe diarrhea with some antibiotic resistant gene

Abstract

Introduction: A total of (116) diarrhea-sufficient patient samples gathered from February 15, 2019 to April 15, 2019. Methodology: Using colonial morphology, microscopic examination, biochemical testing, and the 20 R API Enter systems, the bacteria were able to be identified. Ultimate "PCR" identification of E. coli ("MDH" gene). Result: Rendering to the results obtained according to the morphological, cultural and biochemical characters From the 116 clinical specimen only 42(59.4%) isolates were belonged to E. coli. PCR analysis of the MDH gene (392bp) showed that 26/42(61.9%) were positive. Using the Polymerase chain reaction (PCR) assay the bla-TEM-1 gene11/42(26.19%) of E. coli , were carrying of blaTEM-1 genes was as well as 30/42(71.4%) of E. coli using universal bla-CTX-M primers. Also examined were the antimicrobial susceptibility patterns of the isolates, occurred between 0.00% and 100.00%. Conclusions: The present study revealed that of the rate bacterial infection which causes diarrhea is more causative agent and the higher infection rate among 1month – 2 years age than other age. In this study found the molecular technique method is more efficient than other methods such as (morphology, biochemical and API20E). There was a significant prevalence of ESBL-producing bacterial isolates, and the medicines ciprofloxacin and imipenem were very effective against bacteria isolates. The high prevalence of sul-1 gene than other genes (bla CTX-M and bla TEM).