Genotypic and phenotypic diversity of polyhydroxybutyrate (PHB) producing Pseudomonas putida isolates of Chhattisgarh region and assessment of its phosphate solubilizing ability.

Toshy Agrawal,Anil S Kotasthane,Renu Kushwah

doi:10.1007/s13205-014-0198-9

Toshy Agrawal, Anil S Kotasthane + Show 1 more

Open Access

https://doi.org/10.1007/s13205-014-0198-9

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Journal: 3 Biotech	Publication Date: Feb 19, 2014
Citations: 40	License type: CC BY 2.0

Affiliation: Indira Gandhi Agricultural University

Abstract

A diverse and versatile spectrum of metabolic activities among isolates of fluorescent Pseudomonas putida indicates their adaptability to various niches. These polyhydroxybutyrate producing and phosphate solubilizing isolates showed a high level of functional and genetic versatility among themselves. One of the potential P. putida isolate P132 can contribute as a candidate agent for both biocontrol and PGPR applications. Identified as one of the most efficient PHB producer and phosphate solubilizer, in vitro detection of P132 showed the presence of genes for phenazine, pyrrolnitrin, pyoluteorin and 2,4 diacetylphloroglucinol along with polyhydroxyalkanoate.

Highlights

Pseudomonas putida are ubiquitous bacteria frequently present in water, in soils, and especially in the plant rhizosphere (Timmis 2002; Dos Santos et al 2004)
One of the potential P. putida isolate P132 can contribute as a candidate agent for both biocontrol and PGPR applications
Identified as one of the most efficient PHB producer and phosphate solubilizer, in vitro detection of P132 showed the presence of genes for phenazine, pyrrolnitrin, pyoluteorin and 2,4 diacetylphloroglucinol along with polyhydroxyalkanoate

Summary

Introduction

Pseudomonas putida are ubiquitous bacteria frequently present in water, in soils, and especially in the plant rhizosphere (Timmis 2002; Dos Santos et al 2004). Apart from primary and secondary metabolite production, certain fluorescent Pseudomonads (especially P. putida) are suitable as whole-cell biocatalyzers for the production of several value-added industrial compounds such as biodegradable and biocompatible polyesters called polyhydroxyalkanoates (PHA) or polyhydroxybutyrates (PHB). It accumulates as discrete granules and is used as storage material for carbon and for reducing equivalents by P. putida. Multidisciplinary application of fluorescent Pseudomonads makes it significant and essential to study their phenotypic diversity along with genotypic variability This will be helpful in designing strategies to use the indigenous isolates as bio-inoculants for biocontrol as well as plant growth promotion. The overall aim of the present investigation was to exploit and have a better understanding of beneficial activities of P. putida isolates

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Results and discussion

Conclusion