Abstract
We report a blaNDM-carrying ST405 Escherichia coli recovered from the abdominal fluid of a patient in Shandong, China. This strain belonged to the high-risk phylogenetic group D and carried the virulence genes, papG II, papG III, papC, and iroN. In addition to blaNDM-1, this isolate carried the quinolone resistance gene acc(6′)-Ib and extended-spectrum β-lactamase (ESBL) genes blaCTX-M-15 and blaCTX-M-14. blaNDM-1 was located on a 41 Kb IncN2 self-transmissible plasmid. The IncN2 plasmid named as pJN24NDM1 was fully sequenced and analyzed. Genome comparative analysis showed that IncN2 plasmids harboring carbapenem-resistance genes possessed conserved backbones and variable accessory regions. Phylogenetic analysis of 87 IncN plasmids based on orthologous genes indicated that 9 IncN2 plasmids fell into one phylogenetic clade, while 4 IncN3 plasmids were in two phylogenetic clades of the 74 IncN1 plasmids. The presence of IncN2 plasmids harboring blaNDM in the high-risk clone ST405 E. coli raises serious concerns for its potential of dissemination.
Highlights
New Delhi metallo-beta lactamase (NDM)-producing Enterobacteriaceae have emerged as a critical public-health problem worldwide (Ishii et al, 2017; Livorsi et al, 2018)
Nationwide surveillance of clinical carbapenemresistant Enterobacteriaceae in China showed that NDM production was predominantly associated with carbapenem resistance in Escherichia coli (Zhang et al, 2017; Li et al, 2018; Wang et al, 2018)
We report a ST405 E. coli isolate producing blaNDM−1 harbored by an IncN2 plasmid recovered from a patient with intra-abdominal infection in our hospital
Summary
New Delhi metallo-beta lactamase (NDM)-producing Enterobacteriaceae have emerged as a critical public-health problem worldwide (Ishii et al, 2017; Livorsi et al, 2018). A study from the French National Reference Center showed that 21% of 140 carbapenem-resistant isolates produced NDM carbapenemase (Gauthier et al, 2018). Nationwide surveillance of clinical carbapenemresistant Enterobacteriaceae in China showed that NDM production was predominantly associated with carbapenem resistance in Escherichia coli (Zhang et al, 2017; Li et al, 2018; Wang et al, 2018). In other countries such as India, Pakistan, and the Balkan countries, infections caused by NDM-producer has created large medical and economic burdens (Otter et al, 2017). Targeted resistance surveillance and interventions are needed to control the expanding global distribution of blaNDM-carrying strains
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